Part:BBa_K180004

Game of Life - Primary plasmid

Composite part, consisting of BBa_K180005 and BBa_K180006, but with BBa_K180005 reading along reverse strand, and BBa_K180006 reading along the forward strand.

BBa_K180005 reads along reverse strand, and BBa_K180006 reads along the forward strand to ensure the constitutive promoter BBa_J23119 (present in BBa_K180005) has no effect over LacI or GFP-LVA expression in BBa_K180006.

LacI and GFP-LVA are generated under lux pR regulation, whilst LuxR is constitutively produced.

Usage and Biology

This part has been specified as a basic BioBrick, due to some of the component parts reading in reverse (unable to specify using the component BioBrick format).

To function in the Game of Life design by Team:Southampton, the part has to be used in conjunction with K180007.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 945
  Illegal NheI site found at 968
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 2215
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 985
  Illegal BsaI.rc site found at 2891